Simply drag and drop or upload a pdf document to reduce the size and make it simpler to work with. Oral mucosa is composed of stratified squamous epithelium overlying a connective tissue proper, or lamina propria, with possibly a deeper submucosa figure 91. Filaggrin is a keratin binding protein present in lower layers acidic cytokeratins and 2. The aim of this study was to investigate the expression patterns of cytokeratins cks in dysplastic oral epithelia, to identify useful double immunostaining diagnostic markers. The native ge was reported to express the cytokeratins 5 and 14 and occasionally ck19 in the basal layers. Immunohistochemically, the classical aot phenotype is characterized by a cytokeratin ck profile similar to follicular cyst andor oral or gingival epithelium. Thus a specific cytokeratin expression profile allows the identification of epithelial cells. In contrast, the simple and glandular epithelia express the smaller cytokeratins 7, 8 and 18. Oral squamous cell carcinoma oscc and canine acanthomatous ameloblastoma caa represent two epithelium. Cytokeratins are a family of 20 members which are present in normal epithelial. We have investigated the involvement of these cytokeratin subtypes in the proliferative and migratory behaviour of cultured hrpe.
Our pdf compression tool quickly reduces the size of your pdf file so its easier to share. Expression of cytokeratins in early neoplastic epithelial. The article discusses dental research which examines the keratinization of oral mucosa. Cytokeratins and 19 labelled squamous differentiation or epithelial cells near the surface epithelium, and ck7 had variable expression. The immunohistochemical detection of different cytokeratins cks polypeptides and vimentin has made it easier to explain the histogenesis of many epithelial diseases. In this study, we performed immunohistochemical analysis of cytokeratins cks to examine differences in the lining epithelium of dmcs, edmcs, oocs, and kcots. The assessed value is at a higher percentage of value than. Associated with these ree changes is breakdown of the connective tissues intervening between the tooth and oral cavity, and proliferation in the basal layer of oral epithelium. Protein and mrna expression of simple epithelial keratins in normal, dysplastic, and malignant oral epithelia. Orthokeratinized oral epithelium, such as the palate, contains keratins 1 and 10, whereas gingiva and parakeratinized palatal epithelium contains keratins 1 and 10 or keratins 4 and. On the contrary, decreasing of ck expression is found in dysplastic lesions in the oral epithelium and associated with the development of oscc and oral leukoplakia ohta et al. Oral mucosal epithelial cells grown on porous silicon. Primary tumors and metastases of a given carcinoma share the same pattern of cytokeratins, that distinguishes them from other types of carcinomas, thereby allowing differentiation between the different tumors ref.
They are an important component of intermediate filaments, which help cells resist mechanical stress. In agreement with the concept of changes of cytokeratin expression during stratification are reports that the simple. Immunohistochemically, the classical aot phenotype is characterized by a cytokeratin ck profile similar to follicular cyst andor oral or gingival epithelium based on positive staining with ck5. Immunohistochemical staining patterns of cytokeratins, 14, and 17 in oral epithelial dysplasia including orthokeratotic dysplasia. Expression of cytokeratins in enamel organ, junctional. Cytokeratin cytokeratins are also known as keratins and they are classified as intermediate filament proteins and are found with in the cytoplasm of all epithelial cells 32. Many studies have shown that human gingival keratinocytes grown in submerged culture fail to attain optimal differentiation. The present study aimed to describe the immunohistochemical. Jan 01, 2003 neoplasms and tumours related to the odontogenic apparatus may be composed only of epithelial tissue or epithelial tissue associated with odontogenic ectomesenchyme. Cytokeratin expression in palatal and marginal mucosa of. Cytokeratins in normal lung and lung carcinomas springerlink. Association of cytokeratin 17 expression with diverentiation. The gingiva expresses a great complexity of cytokeratins, including.
Oral mucosa almost continuously lines the oral cavity. Cytokeratin 818 expression indicates a poor prognosis in. Nonkeratinized epithelium, including esophagus, contains keratins 4 and 31, 32. Cytokeratin and vimentin expression in normal epithelium. Cytokeratins as epithelial differentiation markers in. Sep 15, 2009 clinical and histopathologic prognostic implications of the expression of cytokeratins 8, 10, 14, 16, 18 and 19 in oral and oropharyngeal squamous cell carcinoma rima a. Epitheliumcytokeratins are epitheliumspecific derived tumors maintain the expression of intermediate filaments with diameters many cytokeratin polypeptides characteristic of. Several types of keratins have been described and their potential role as molecular markers in various malignancies has been investigated for different thyroid tumors.
Cytokeratin and vimentin expression in normal epithelium and. During malignant transformation of epithelial cells, the cytokeratin expresssion patterns changed, depending on the differentiation grade of the. Posteriorly soft palate which is attached to the posterior border of the hard palate and projects posteriorly in to the pharynx, separating its oral and nasal parts. Figure 91 general histological features of an oral mucosa composed of stratified squamous epithelium overlying. The keratin expression pattern in oral stratified epithelium is related to the cellular differentiation level. Stratified squamous epithelium devidid in to two parts anteriorly is the hard palate which forms the partition between the nasal and the oral cavities. Usually, to split a pdf you have to spend a lot of money on premium software. This study reports an in vitro culture system for oral gingival epithelial cells, in which they are grown at the airliquid interface, on polycarbonate inserts, in the presence of an nih3t3 feeder layer. Expression of intermediate filament proteins in benign lesions of the oral mucosa eur arch otorhinolaryngol. The oral epithelium, like other covering and lining epithelia, maintains its structural integrity by a process of continuous cell renewal in which cells produced by mitotic divisions in the deepest layers migrate to the surface to replace those that are shed nakamura et al. Expression of cytokeratins in the epithelium of canine. Effect of snuff on cytokeratin expression in oral vestibular sulcus epithelium j oral pathol med. You can select pages in the visual interface or just type in the number of the pages you want to extract.
Correspond ingly, immunocytochemical studies using several monoclo nal antibodies specific for the simple epithelial cytokeratins. Ouhayoun jp, gosselin f, forest n, winter s, franke ww. All adenocarcinomas of the lung reveal a simple epithelium type cytokeratin pattern nos. Neoplasms and tumours related to the odontogenic apparatus may be composed only of epithelial tissue or epithelial tissue associated with odontogenic ectomesenchyme.
In contrast, cki3 is expressed in normal nonkeratinized oral epithelium as well as in head and neck cancers 6. Cytokeratins are a class of intermediate filaments used to demonstrate epithelial differentiation. Cytokeratins, which are important components of the cytoskeleton, are excellent epithelial differentiation markers used to study neoplastic and inflammatory diseases. Fusion between the oral epithelium and ree follows, with. Their altered expression is evident in various benign, hyperplastic and neoplastic lesions.
Porphyromonas gingivalis fimbriae bind to cytokeratin of. A variety of cell surface structures have been postulated to play a role in the interaction of p. Fusion between the oral epithelium and ree follows, with breakdown of the central area of the epithelium. Heyden a, huitfeldt hs, koppang hs, thrane ps, bryne m, brandtzaeg p. Five classes of intermediate filaments have been described 1. Expression of cytokeratins in the epithelium of canine odontogenic tumours article in journal of comparative pathology 1454. Cell proliferation markers bromodeoxyuridine and proliferating cell nuclear antigen and the cytokeratins. Details about the cellular elements in saliva and the keratinization of epithelial cells are provided. Expression of simple epithelial type cytokeratins in. Interspecies variations in oral epithelial cytokeratin expression. Polyclonal and monoclonal antibodies to cytokeratin polypeptides were used to study the expression of these intermediate filament proteins in normal, squamous metaplastic, and neoplastic epithelium of the uterine cervix, in order to investigate the morphogenesis of early epithelial changes preceding cervical squamous cell carcinoma. Thus they are used clinically to identify the cell of origin of various human. Porphyromonas gingivalis plays an important role in the initiation and progression of periodontal disease. Cytokeratin alteratin in oral leukoplakia and oral squamous cell carcinoma article pdf available in oncology reports 183.
The expression pattern of cytokeratins in human normal tongue dorsal mucosa, gingiva, and buccal mucosa was examined immunohistochemically with 9 kinds of antikeratin monoclonal antibodies ae3, ae5, 903, no56, n. The aim of this study was to determine the degree to which the epidermis and oral epithelium of species other than man express cytokeratin ck intermediate filaments, which are markers of epithelial differentiation. We would like to show you a description here but the site wont allow us. Moll r, franke ww, schiller dl, geiger b, krepler r. In addition, we carried out immunohistochemical examination of langerin to clarify the biological characteristics of the orthokeratinized lining epithelium of dmcs, edmcs, and oocs. Differences in the expression of cytokeratins ck in specimens obtained from snuff. Immunohistochemical localization of cytokeratins in the. Ringel, in advances in molecular and cellular endocrinology, 2006. Expression of cytokeratin in the epithelium of dentigerous. The expression of cytokeratins 18 and 19 by human retinal pigment epithelial cells hrpe has been suspected of being associated with hrpe proliferation.
The normal pattern shows the keratin pair k5 and k14 in the stratum basale whereas k1 and k10, or k4 and k, are the two pairs associated with differentiating suprabasal cells. Use in basic research ck structure and classification weight in stratified epithelium, and high cytokeratin expression in skin epithelium, ytokeratins ck are the molecular weight cytokeratins 67 kda in normal oral epithelia and respiratory epithelia. The cytokeratin expression in skin epithelium, normal oral epithelia and respiratory epithelia has been well investigated. Biology of oral mucosa and esophagus jnci monographs. Human odontogenic keratocyst transplants in nude mice. Increased expression of cytokeratins ck8 and ck19 is. Bovine alveolar cells also differ from cells of the tracheal epithelium by the absence of a basic cytokeratin polypeptide. In this study, we seeked to define the prognostic potential of cytokeratin and vimentin expression patterns in squamous cell carcinomas sccs of the oral cavity. The assessment is unequal for the following reason. Antibodies against cytokeratin are most commonly used in the diagnosis of metastatic carcinomas, but can also be used to identify primary epithelial or epitheliumcontaining cns neoplasms, such as craniopharyngiomas, chordomas, teratomas, epithelial cysts, and choroid. Contents definition structure and function classification cell polarity apical domain,lateral domains, basal domains gland epithelial cell renewal oral epithelium 3. Therefore, in the present study we examined the expression of cytokeratins in the three types of epithelia to investigate the epithelial phenotypes. During malignant transformation of epithelial cells, the cytokeratin expresssion patterns changed, depending on the differentiation grade of the carcinomas.
Cytokeratins and retinal epithelial cell behaviour. In a variety of organs, the expression of distinct intermediary filaments are associated with patient prognosis. Cytokeratins are integral structural proteins of benign and malignant epithelial cells. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. In the suprabasal layers, the native ge expresses ck 110 correspondence. Fixed, waxembedded samples of skin, buccal mucosa and gingiva from rhesus monkey, marmoset, cow, sheep, pig, ferret. Coordinate expression of cytokeratins 7 and 20 in feline and canine carcinomas a. All adenocarcinomas of the lung reveal a simpleepitheliumtype cytokeratin pattern nos. To study the profile of cytokeratins in olp and their possible association with dysplastic alterations, monoclonal antibodies were used for cytokeratins 10, 14 and 19, in 26. The oral epithelium is stratified squamous type consisting of cells called keratinocytes.
Immunohistochemical staining patterns of cytokeratins, 14. Interspecies variations in oral epithelial cytokeratin. Aug 02, 2014 differentiationdependent expression of keratins in human oral epithelia j invest dermatol. Intermediary filaments are involved in cell motility and cancer progression. Its role and expression profile in oral health and disease objective this article summarizes overview of cytokeratins in relation to fetal and adult epithelium along.
In vitro reconstruction of human junctional and sulcular. Immunohistochemical staining patterns of cytokeratins. Although the extruded membranecoatinggranule contents fuse to form multiple broad lipid sheets in the intercellular spaces of the stratum corneum of this tissue, the number of individual lamellae in oral tissue. Cytokeratins are keratin proteins found in the intracytoplasmic cytoskeleton of epithelial tissue. The expression of calretinin and cytokeratins in canine. Defined subsets of individual cytokeratin pairs are characteristically expressed, depending on the type of epithelial cell tissue and level of differentiation. These cells undergo a process of maturation during which the cells produced by the mitotic division in the basal layer migrate to the surface where they are shed off and are replaced by the maturing cell population. Monoclonal and polyclonal antibodies to individual keratins k10, k and k14 were used in a two. Cytokeratins and odontogenesis patterns of expression of cytokeratins in the odontogenic epithelium have been described in a very general way. Cytokeratins in epithelia of odontogenic neoplasms, oral.
Differences in cytokeratin synthesis in gingival epithelium and the adjacent alveolar mucosa. The epithelium exhibits cornification in its upper portions, including squame for mation see also the region marked by the horizontal bracket on the upper right of a. In the study, mucous membrane smears were examined in order to identify they cytologic detail of epithelial cells. Hema keswani rajiv gandhi college of dental sciences,bangalore 2. Oral mucous membrane free download as powerpoint presentation. Immunocytochemistry revealed the detection of epithelial cytokeratins and 14, col iv and ki67 in the reconstituted oral mucosa model. Material and methods the maxillae of wistar rats, 1, 2, 3 and 7 wk of age, were used, and the expression of ck14, ck17, ck19, ck10ck and ae1ae3 was detected using immunoperoxidase techniques.
In conclusion, the typical if of odontogenic epithelium was ck14, while ck8, 10 and 18 were absent. Importantly, they are tools in diagnostic pathology, most importantly in the detection of tumors. Differentiationdependent expression of keratins in human oral epithelia j invest dermatol. In keratinized oral epithelium, about 50% of the intercellular space of the stratum corneum is occupied by desmosomes, and the interdesmosomal regions are frequently dilated. Expression of these cytokeratins within epithelial cells is largely specific to particular organs or tissues. Odontomas exhibited an immunohistochemical profile similar to that of the dental germ. Cytokeratins play an important structural and protective role in maintaining the integrity of the epithelium of the anterior segment of the eye. The immunohistochemical expression patterns of cytokeratin polypeptides and vimentin were investigated in normal epithelia and squamous cell carcinomas of the larynx with special emphasis on tumor grading. This allows the emergence of the tip of a tooth into the oral cavity through an epithelial lined channel, thus ensuring no loss of epithelial continuity.
The expression of heat shock proteins hsp60 and hsp70 and cytokeratins ck110 and ck718 were compared in epithelium of oral lichen planus olp lesions and oral fibromas using an avidin. Epithelium of oral mucosa protective function barrier and dehydration, absorptive function, 3 types non keratinized stratified squamous epithelium, orthokeratinized stratified squamous epithelium, and parakeratinized stratified squamous epithelium. Cytokeratins as epithelial differentiation markers. Pdf cytokeratin alteratin in oral leukoplakia and oral. Cytokeratins in epithelia of odontogenic neoplasms. Likewise, ck8, which is expressed in simple epithelium 6, 28, is not expressed in normal oral mucosa cells but may be expressed in carcinomas 24. Distribution of cytokeratins type i acidic type ii basic low molecular wt high molecular wt. In contrast, squamous cell carcinomas of the lung contain an unusual complexity of cytokeratins.
Disposition of cytokeratins from the cytoplasmic plaque toward the nuclear periphery. Cytokeratins ck are type i and type ii intermediate filaments 2, 3. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Cytokeratins as epithelial differentiation markers in premalignant and malignant oral lesions. Antibodies against cytokeratin are most commonly used in the diagnosis of metastatic carcinomas, but can also be used to identify primary epithelial or epithelium containing cns neoplasms, such as. Study on expression pattern of cytokeratins in human. The expression of cytokeratins cks and calretinin has been previously established in the canine tooth bud and odontogenic tumours. Comparative study of cytokeratin and langerin expression in. Daniela elena costea, the gade institute, faculty of medicine and dentistry, university of bergen, n5021 bergen, norway. Cytokeratins gingival epithelium is characterized by intermediate filament called cytokeratins, responsible for maintenance of homeostasis of keratinocytes. Barrier function and cytologic features of the ocular surface epithelium after autologous cultivated oral mucosal epithelial transplantation. Introduction oral mucosa gingiva keratinization gingival keratinization process control of keratinocyte differentiation keratinized vs non keratinized epithelia cytokeratins of oral mucosa.
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